返回列表 發帖

聚左旋乳酸PLLA生物相容性


聚左旋乳酸PLLA生物相容性
The study of PLLA biocompatibility
中文摘要

植入體內的PLLA,細胞必須要先能附著在其表面上,進行增生,進一
步分化而行使正常功能。因此,本實驗也探討細胞對PLLA 試片的附著與增生能
力,以polystyrene culture disc 作為控制組,另外並以接觸角測定儀與表面
粗度儀來測量PLLA 試片與polystyrene culture disc 的親水性與表面粗糙
度。敏感性測試與基因毒性測試則分別是以天竺鼠與特定的沙門氏菌來研究試片
的致敏感性與致突變性。實驗結果顯示,即使是最高濃度的PLLA 萃取液與降解
產物,均不會產生任何的細胞毒性、基因毒性與致敏感性。PLLA 試片表面與水
的接觸角以及的粗糙度分別為70℃與0.51μm,而控制組polystyrene
culture disc 則為50℃與0.28μm。細胞在PLLA 試片上的附著能力略優於控
制組(p<0.05),附著率從加入細胞後12 小時以前的2.5x102 / hr,增加到
12 小時以後2x103 / hr,而兩者的增生能力則是無統計上之差異。本實驗所
採用的PLLA 試片具有不錯的生物相容性,未來應用在各種不同的醫療用途是深
具潛力的。聚左乳酸(PLLA)的應用相當廣泛,從以前工業用的罐子,不織布,至今已發展
應用到生物醫學方面,如縫線,牙周再生膜,手術骨釘骨板等等。PLLA 分子量
越高,機械強度越強,因此適合用於手術固定用之骨釘骨板的固定,但缺點是在
人體內的降解時間就越久;而較低分子量的PLLA,雖然降解較快,卻因為機械
強度較差的關係,則多使用在受力較小的部位。本實驗室與某生技公司所合力開
發出具商業化潛力的低分子量(140-160KD)PLLA 試片,擁有與高分子量PLLA
相當的機械強度。為了了解本PLLA 試片在活體內降解的過程中是否會引起某些
層面的生物反應,本實驗將對PLLA 試片以及其降解產物對生物細胞、過敏反應
以及基因突變的影響來做相關的探討。為了模擬材料在活體內的環境,本研究欲
檢測PLLA 萃取液、降解產物,以及PLLA 試片對3T3 fibroblast 是否具有細
胞毒性。

英文摘要
Since PLLA (poly-L-lactic acid) was produced by heating lactic acid under vacuum in
1932, it has been used in biology and medicine. PLLA possesses many desirable
properties that make it advantageous for many medical applications, such as sutures,
surgical bone plates, GTR membranes, etc. The aim of this study was to evaluate the
short-term biocompatibility of a low-molecular-weight (140-160KD) PLLA disc
which has a good mechanical strength. The effects of 1-, 3-, 5-day PLLA extracts and
its artificial degradation products obtained by heating at 90℃for 30hrs on 3T3
fibroblast were studied by MTT assay. The abilities of the PLLA disc surface to allow
cell attachment and subsequent proliferation were also evaluated. In addition, the
surface roughness and wettability were examined by mechanical surface profilometer
and contact angle meter, respectively. The abilities of PLLA extracts and degradation
products to cause allergic reaction on guinea pigs were detected by sensitization test
(Buehler test), and their mutagenic potential were performed by Ames test using
Salmonella typhimurium as test strains. The PLLA disc, PLLA extracts and
degradation products did not show cytotoxic, allergic, or genotoxic activities. The
PLLA surface roughness and the contact angle measured were 0.51μm and 70℃, and
the polystyrene culture disc were 0.28μm and 50℃. The surface roughness of PLLA
disc seemed to facilitate cell attachment, but the subsequent proliferation has no
difference as compared to the polystyrene culture disc. These results suggest the
low-molecular-weight PLLA used in this study may be regarded as a promising
biomedical material.


分享到Twitter
分享到FunP
分享到Plurk噗浪
分享到UDN
分享到FaceBook

聚左旋乳酸PLLA這篇感覺太專業了,可能一般人不會想知道這個東西~
整形美容達人在此,不論是臉部整形或是雷射整形都可問我!

TOP

返回列表